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Use of Potato Virus X (PVX)-Based Vectors for Gene Expression and Virus-Induced Gene Silencing (VIGS)
Potato virus X–based vectors are a well established system for rapid in planta studies. The vectors can be used for expression of proteins in plants and to down‐regulate genes through virus‐induced gene silencing. The development of binary‐based vectors for Agrobacterium delivery makes this system well suited to high‐throughput studies. Protocols are given for establishing infections to achieve expression and VIGS through in vitro transcription and Agrobacterium delivery to glasshouse and in vitro–grown plant material. Curr. Protoc. Microbiol. 8:16I.1.1‐16I.1.13. © 2008 by John Wiley & Sons, Inc.
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